Karslı T. (Yürütücü)
Yükseköğretim Kurumları Destekli Proje, 2018 - 2019
Playing
a key role in immunity and autoimmunity, Major Histocompatibility Complex (MHC)
contains microsatellite regions and polymorphisms associated with resistance to
several diseases and thermophysiologic characteristics in farm animals. This
study aims to reveal genetic diversity in four native Turkish goat populations
via MHC related gene regions including MHC-linked microsatellite markers (BF1,
BM1818, BM1258, SMHCC1 and DYMS1) and MHC Class II-DRB gene. A total of 120
unrelated animals belonging to Hair (HAI), Honamlı (HNM), Kabakulak (KBK) and
Norduz (NRD) from different representative populations in Antalya and Van provinces
were sampled and genotyped for molecular analysis. Based on MHC-linked
microsatellite markers, number of alleles ranged from 8.20 (NRD) to 8.80 (HAI
and KBK) across studied goat populations. Observed heterozygosity was between
0.68 (NRD) and 0.80 (KBK), whereas expected heterozygosity ranged from 0.74
(NRD) to 0.82 (KBK) in native Turkish goats. Inbreeding coefficients were 0.04,
0.13, -0.01 and 0.09 for HAI, HNM, KBK and NRD populations, respectively. A 284
bp length PCR products belonging to MHC Class II-DRB gene region were digested
separately with PstI, TaqI, BsaHI and AluI restriction endonucleases to assess
polymorphism status together with HardyWeinberg equilibrium in studied goat
populations. P allele frequency ranged from 0.73 (KBK) to 0.95 (NRD), while p
allele frequency was between 0.05 (NRD) and 0.27 (KBK) in PstI polymorphism.
The highest and lowest frequency of T allele were detected in HNM (0.80) and
KBK (0.49), respectively, whereas frequency of t allele was between 0.20 (HNM)
and 0.51 (KBK) in TaqI polymorphism. G and A allele frequency were between 0.16
(HNM) - 0.39 (HAI) and 0.84 (HNM) – 0.61 (HAI), respectively in BsaHI
polymorphism, while three different genotypes and two alleles, which were
different from results reported in the literature, were observed in AluI
polymorphism. In this study, high genetic diversity and low inbreeding were
detected in native Turkish goats according to MHC-linked microsatellite
markers. Similarly, native Turkish goats hold enough polymorphisms in MHC Class
II-DRB gene which gives opportunity to support selection strategies against
tuberculosis and heat stress in the future.