Akademik Veri Yönetim Sistemi
Kılıç F. S. (Yürütücü)
Yükseköğretim Kurumları Destekli Proje, 2016 - 2016
Abstract
Objective: Pregabalin (PGB) is a compound used in the treatment of
epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that
PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory
effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor
necrosis factor-alpha (TNF-alpha) and interleukine-1 beta (IL-1 beta) acting as
acute phase cytokines in inflammation, and antiinflammatory cytokine IL-10, in
rats.
Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and
indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control
group were injected once intraperitoneally prior to administration of 100 mu l
of 1% CAR into the right hind paw of the rats. The paw thickness was measured
using gauge calipers (Vernier calipers) before (0 hour) and every hour
afterwards for 6 hours following the inflammation induction. The cytokine
levels in the blood serum obtained intracardiacally were determined after 6
hours using the enzyme-linked immunosorbent assay method. p < 0.05 was
considered statistically significant.
Results: There was no significant difference between the 0 and 6th hour
considering the paw thickness in all groups, except in the CAR group. CAR
significantly increased the paw thickness at 6 hours compared to the 0 hour.
All doses of PGB and INDO significantly reduced the paw thickness after 6 hours
compared to the CAR group. The TNF-alpha and IL-1 beta levels in the PGB and
INDO groups were comparable to the control group, whereas in the CAR group,
these levels were increased. The IL-10 level was enhanced in the PGB 50 mg/kg
and INDO groups.
Conclusion: It was observed that all doses of PGB exerted
anti-inflammatory-like effects comparable to INDO, supported by their effects
on the levels of cytokines.