Dye decolorization of magnetically retrievable formulation of laccase with amine-functionalized microparticles


KORKMAZ F., NURAL YAMAN B., Gedikli S., AYTAR ÇELİK P., DEMİRBİLEK M., ÇABUK A.

JOURNAL OF MICROBIOLOGICAL METHODS, vol.211, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 211
  • Publication Date: 2023
  • Doi Number: 10.1016/j.mimet.2023.106755
  • Journal Name: JOURNAL OF MICROBIOLOGICAL METHODS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Veterinary Science Database
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Laccase is an important enzyme used in many industries because of its multi-substrate catalyst. New immobi-lization agents are excellent tools for enhancing the abilities of this enzyme. In this study, immobilization of laccase on silica microparticles with NH2 (S-NH2) surface modification to use in dye removal applications was aimed. The yield of immobilization by this method was found to be 93.93 & PLUSMN; 2.86% under optimum conditions. In addition, this newly created immobilized enzyme was adapted to a decolorization application with 87.56 & PLUSMN; 1.60% efficiency. Silica microparticles with NH2 (S-NH2) surface modification were used for laccase immobili-zation and this immobilized laccase had quite good potential. Besides, Random Amplified Polymorphic DNA (RAPD) analysis in evaluating the toxicity of the decolorization process was utilized. After amplification with two RAPD primers, decreased toxicity of dye in this study was observed. This study showed that RAPD analysis in toxicity testing could be accepted as an alternative and practical method that this approach will contribute to the literature in terms of providing fast and reliable results. The use of amine-modified surface silica microparticles for laccase immobilization and RAPD for toxicity testing is a crucial aspect of our investigation.