Akademik Veri Yönetim Sistemi
4th INTERNATIONAL EURASIAN MYCOLOGY CONGRESS , Çanakkale, Türkiye, 3 - 05 Eylül 2024, ss.115, (Özet Bildiri)
Screening of Fungi Isolated from Hypersaline Environments for EPS Production and Production Optimization with Selected Isolate Penicillium allii
Various stress tolerance mechanisms are responsible for the adaptation of microorganisms to extreme conditions, and they develop defense methods by producing different metabolites. Polysaccharides, one of the metabolites produced by fungi adapted to extreme conditions, attract biotechnological interest due to their diversity and different molecular structures. In this study, 62 halophilic/halotolerant fungal isolates from Acıgöl and Çamaltı Salt Pans were screened for EPS production, and medium optimization was performed for the selected isolate. After 7 days of incubation in liquid medium, EPS in the supernatant was separated by cold ethanol extraction. The carbohydrate structure of the obtained EPS was confirmed by the phenol-sulfuric acid method. EPS production conditions for Penicillium allii, identified as halophilic by halotolerant testing, were optimized using the Box-Behnken experimental design. According to the design, which examined 5 factors at 3 levels from the medium components, there was a linear relationship between EPS production and the highest concentrations of carbon, nitrogen, and phosphate sources used in the study. For NaCl concentration, the situation was different, with EPS production increasing at the lower concentration studied. It was suggested that P. allii, due to its halophilic nature, might increase EPS production as a defense mechanism at relatively low salt concentrations. The amount of EPS obtained under optimum conditions showed a 4.5-fold increase compared to non-optimized conditions. The obtained EPS were partially purified by dialysis and analyzed. It was determined that the EPS contained 99.4% carbohydrate, 0.024% protein, and 0.53% uronic acid. Metabolites produced by fungi adapted to extreme conditions, especially exopolysaccharides, show biotechnological promise due to their diversity and different molecular structures.
Keywords: Exopolysaccharide, halophilic fungus, Box-Behnken.