The effect of baicalein on endoplasmic reticulum stress and autophagy on liver damage

ÜSTÜNER M. C., TANRIKUT C., Üstüner D., Kolaç U., Köroğlu Z. Ö., Burukoğlu D., ...Daha Fazla

HUMAN & EXPERIMENTAL TOXICOLOGY, cilt.40, sa.10, ss.1624-1633, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 10
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1177/09603271211003634
  • Dergi Adı: HUMAN & EXPERIMENTAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, Chimica, CINAHL, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1624-1633
  • Anahtar Kelimeler: Baicalein, endoplasmic reticulum stress, autophagy, CCl4, hepatic damage, CARBON-TETRACHLORIDE, CELL-DEATH, ER-STRESS, CASPASE ACTIVATION, INDUCED APOPTOSIS, CANCER CELLS, INHIBITION, HEPATOTOXICITY, MECHANISMS, INJURY
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Carbon tetrachloride (CCl4) is a toxic chemical that causes liver injury. CCl4 triggers endoplasmic reticulum (ER) stress and unfolded protein response (UPR). UPR triggers autophagy to deal with the damage. The aim of this study was to investigate the effect of baicalein, derived from Scutellaria baicalensis, on CCl4-induced liver damage concerning ER stress and autophagy. Two groups of Wistar albino rats (n = 7/groups) were treated with 0.2 ml/kg CCl4 for 10 days with and without baicalein. Histological and transmission electron microscopy (TEM) analysis, autophagy, and ER stress markers measurements were carried out to evaluate the effect of baicalein. Histological examinations showed that baicalein reduced liver damage. TEM analysis indicated that baicalein inhibited ER stress and triggered autophagy. CCl4-induced elevation of C/EBP homologous protein (CHOP), glucose-regulating protein 78 (GRP78), activating transcription factor 4 (ATF4), activating transcription factor 6 (ATF6), inositol requiring enzyme 1 (IRE1), pancreatic ER kinase (PERK), and active/spliced form of X-box-binding protein 1 (XBP1s) ER stress markers were decreased by baicalein. Baicalein also increased the autophagy-related 5 (ATG5), Beclin1, and Microtubule-associated protein 1A/1B-light chain 3-phosphatidylethanolamine-conjugated form (LC3-II) autophagy marker levels. In conclusion, baicalein reduced the CCl4-induced liver damage by inhibiting ER stress and the trigger of autophagy.