Detection And Virulence Of Ophiosphaerella korrae Causing Spring Dead Spot Disease in Golf Courses in Antalya


Ünal F., Kurbetli İ.

2nd International Silkroad Congress, Iğdır, Türkiye, 26 - 27 Eylül 2023, ss.110-115

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Iğdır
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.110-115
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Seventy-one diseased plant samples were taken from turfgrass areas during the surveys in Antalya province, Türkiye in 2015. Dead spot symtoms were observed in five golf courses during the surveys conducted in Belek region, in Antalya. On golf courses where the agent is isolated, collapsed small patches in the form of whitish yellow circles 10-12 cm in size were seen. When the roots, stolons and rhizomes of the diseased plants were examined, it was observed that they were browned and rotten. As a result of isolations from infected roots on potato dextrose agar (PDA) supplemented with 100 mg/liter streptomycin sulfate, a fungus with aerial mycelial developed which was light gray at first and turned dark gray over time. As a result of isolations from turfgrass diseases plants, obtained isolates were identified with the rDNA-ITS sequences analysis using universal primer pair ITS1/ITS4. The ITS1-5.8S-ITS2 region of isolates were sequenced and performed BLAST analyzes. Five isolates were identified as Ophiosphaerella korrae. Pathogenicity tests were performed on 8 week old C. dactylon variety grown in plastic pots (12 × 12 cm). The substrate (sandy soil and perlite) was infested separately with five isolates of O. korrae grown on autoclaved wheat kernels, using 10 infested kernels per pot. As a result of the pathogenicity tests on Petri dishes and pots, diseases severity value of the  isolates in Petri dishes were range from 92%- 98%, were range from in pots 82%- 95% . This is the first report of Ophiosphaerella korrae causing root rot of turfgrass areas in Türkiye.