Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.193, 2023 (SCI-Expanded)
In this investigation, the Leishmania spp., was sensitively detected via a label-free electrochemical-based oligonucleotide genosensing assay based on the performing hybridization process. The multiple electrochemical modifier-tagged agents were impressively applied to improve the electrochemical responses. However, a thermionic vacuum arc has been used which is a time-consuming, high vacuum regime, low surface roughness, high quality, and plasma-based technique for coating nano-dimension materials and providing an appropriate surface for electrochemical investigations. Using this research designed genosensing bio-platform, the low detection and quantification limits (LOD and LOQ) have been reached to the minimum value of 1.12 fM and 3.55 fM, respectively. The potency of this electrode was elucidated due to working in the wide linear range from 100 nM to 10 fM. 1, 2, and 3-base mismatched sequences and the high selectivity and performance of the engineered assay in the negative control samples were evaluated. Importantly, the recoveries and RSDs values were 98.2 –103.5% and 2.7–3.4%, respectively. The repeatability and reproducibility of the aforementioned bio-assay have been clarified. Finally, according to the literature review and best of our knowledge, the method reported in this work was the most suitable and novel pathway for rapid and quantitative detection of Leishmania spp., for human serum samples.