IDENTIFICATION OF PATHOGENS IN PEDIATRIC SEPTIC ARTHRITIS CASES BY REAL-TIME PCR: A MULTI-CENTER STUDY IN TURKEY (PEDSART STUDY)


İşerinepesov M., Kılıç Ö. , Sali S., Yeşil E., Akar A., Kaman A., ...More

The 39th Annual Meeting of the European Society of Paediatric Infectious Diseases (ESPID), Geneve, Switzerland, 24 - 29 May 2021, vol.1, no.272, pp.556

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Geneve
  • Country: Switzerland
  • Page Numbers: pp.556

Abstract

Background: Septic arthritis (SA) is a serious bacterial infection that must be treated efficiently and timely, but the huge number of culture-negative cases makes the local epidemiological data important. Accordingly, the aim of this study was to evaluate the etiology, clinical characteristics, and therapeutic approach of SA in children in Turkey, emphasizing the role of real-time polymerase chain reaction (PCR) techniques in diagnosis.

Methods: In this multi-center, prospective study, children who were hospitalized with the diagnosis of SA between February 2018 and July 2020 in 23 hospitals in 14 cities of Turkey included. Clinical, demographic, laboratory, radiological findings have been noted and real-time PCR have been performed in synovial fluid samples.

Results: Seventy-five children between 3 to 204 months (median 74 months) diagnosed with acute SA were enrolled. The combination of synovial fluid culture and real-time PCR resulted in the detection of causative bacteria in 33 (44%) of patients. In 14 (18.7%) patients, the etiological agent was demonstrated by the PCR method only. The most commonly isolated etiologic agent was Staphylococcus aureus, which was detected in 22 (29.3%) patients, while Streptococcus pyogenes was found in four (5.3%) patients and Kingella kingae in three (4%) patients.Concurrent osteomyelitis was seen in 10/75 (13.3%) children.

Conclusions: Staphylococcus aureus is the main pathogen in pediatric septic arthritis cases and with the use of advanced diagnostic approaches like real-time PCR the chance of diagnosis increases.