OPTIMIZATION AND CHARACTERIZATION OF HALOPHILIC PHB PRODUCTION

Göç C., Nural Yaman B., Mutlu M. B., Çabuk A.

Zeugma 16th International Congress on Scientific Research, Gaziantep, Türkiye, 26 - 28 Haziran 2026, ss.261-262, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Gaziantep
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.261-262
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Polyhydroxybutyrate (PHB) is a biodegradable and biocompatible plastic that has emerged as a promising alternative to reduce environmental pollution caused by petroleum-derived However, the high production costs of PHB limit its industrial-scale application. Therefore, alternative strategies for producing PHB are important. The aim of this study is to produce a high-vield polymer using halophilic bacteria. For this purpose, the isolate was identified using a molecular approach, and then the PHB yield was maximized by optimizing parameters such as carbon source concentration, incubation time, and stirring speed using CCD from RSM. Finally, the PHB produced under optimum conditions was characterized by FTIR, NMR, and TGA. Standard PHB was used as a control. According to 16S rRNA analysis, the isolate was identified as Vreelandella janggokensis. CCD analysis revealed that maximum PHB production was obtained at a carbon source of 20g/L, an incubation time of 72 hours, and a stirring speed of 150 pm. FTIR spectroscopy confirmed the polyester structure with an ester carbonyl peak detected at 1722 cm' and a fingerprint region perfectly matching standard PHB. Furthermore, 1H-NMR findings showed that the polymer has the same molecular backbone as standard PHB and is produced with high purity. Consequently, this optimized production model and the structural analyses performed reveal that the produced PHB possesses suitable purity and thermal stability for other high-value-added applications, particularly drug delivery systems. Moreover, these optimum conditions can be utilized in future scaling processes.

Keywords: Polyhydroxybutyrate (PHB), Halophilic Bacteria, Biopolymer, Optimization