Akademik Veri Yönetim Sistemi
8. International Mediterranean Scientific Research Congress, Antalya, Türkiye, 13 - 15 Ağustos 2025, ss.403-405, (Özet Bildiri)
An important source of extracellular matrix (ECM) for biomedical applications is human umbilical cordderived Wharton's gel (WJ). The high biocompatibility of WJ ECM and its structure that supports cellular interactions is seen as a promising biomaterial for tissue engineering and regenerative medicine applications (1,2,3). This study focused on developing the best protocol for the decellularization of extracellular matrix (ECM) derived from WJ, with a focus on maintaining the structural and biochemical integrity. The decellularization process was carried out by applying enzymatic digestion steps and detergent-based solutions at specific concentrations and durations. This was done with the aim of maximizing both the effective removal of cellular components and the biological activity of the outer layer (ECM). DAPI staining, DNA quantification and histological analyses were used to quantify the effectiveness of the decellularization process. Preliminary investigations show that the decellularized extracellular material (ECM) retains its fibrous structure and biochemical composition. This suggests that it is a promising biomaterial for tissue engineering applications. This study highlights the potential of WJ-derived extracellular matrix (ECM) as a promising scaffold material in regenerative medicine and presents significant findings regarding its capacity to support cellular interactions.