DICER governs characteristics of glioma stem cells and the resulting tumors in xenograft mouse models of glioblastoma

Mansouri S., Singh S., Alamsahebpour A., Burrell K., Li M., Karabork M., ...Daha Fazla

ONCOTARGET, cilt.7, sa.35, ss.56431-56446, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 7 Sayı: 35
  • Basım Tarihi: 2016
  • Doi Numarası: 10.18632/oncotarget.10570
  • Dergi Adı: ONCOTARGET
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.56431-56446
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

The RNAse III endonuclease DICER is a key regulator of microRNA (miRNA) biogenesis and is frequently decreased in a variety of malignancies. We characterized the role of DICER in glioblastoma (GB), specifically demonstrating its effects on the ability of glioma stem-like cells (GSCs) to form tumors in a mouse model of GB. DICER silencing in GSCs reduced their stem cell characteristics, while tumors arising from these cells were more aggressive, larger in volume, and displayed a higher proliferation index and lineage differentiation. The resulting tumors, however, were more sensitive to radiation treatment. Our results demonstrate that DICER silencing enhances the tumorigenic potential of GSCs, providing a platform for analysis of specific relevant miRNAs and development of potentially novel therapies against GB.