Characterization and Pathogenicity of Rhizoctonia AG P Causing Damping-off on Turfgrass.


Ünal F., Çavuşoğlu A.

5th Internatıonal Balkan Agriculture Congress, Edirne, Türkiye, 20 - 23 Eylül 2023, ss.285-291

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: Edirne
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.285-291
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

The genus Rhizoctonia consists of species that have one, two (binucleate) and multinucleate hyphae that are pathogenically, morphologically and genetically different from each other. These species are named and divided into subgroups depending on the anastomosis reactions between their hyphae and they show different pathogenic characteristics according to their hosts. Binucleate Rhizoctonia spp. generally live in soil and plant debris as saprophytes, but some form parasitic relationships with plants. In this study, surveys were conduct in parks in Kocaeli provinces in 2022. As a result of isolations from 52 turfgrass plants, two binucleate Rhizoctonia AG P isolates were identified in accordance with the rDNA-ITS sequences analysis. The rDNA internal transcribed spacer (ITS) regions of the isolates were amplified by polymerase chain reaction (PCR) using universal fungal rDNA primer pair ITS1/ITS4. The ITS1-5.8S-ITS2 region of isolates were sequenced and performed BLAST analyzes. Pathogenicity tests were performed two times in pots using turfgrass seeds in greenhouse conditions. As a result of the pathogenicity tests, it was determined that both isolates cause damping-off on turfgrass.