Akademik Veri Yönetim Sistemi
FIBERS AND POLYMERS, cilt.23, sa.11, ss.3028-3038, 2022 (SCI-Expanded)
Tissue engineering strategies include successful management of bone injuries with biomaterials to develop bone-like structures. The production of natural biomaterials for this purpose helps both to ensure tissue integrity and to present alternative products for clinical purposes. However, natural materials are mostly used together with synthetic or metallic materials due to their low stability and mechanical properties for bone tissue regeneration. There is a clinical need for material combinations that have both antimicrobial properties and the ability to increase osteogenic induction. This work aims to fabricate a nanofiber scaffold using polyurethane (PU) loaded with natural fish head powder (FHP) from Argyrosomus regius species and copper (II) chloride (CuCl2) using the electrospinning technique in order to respond to this clinical need. There has not been any material combination in the literature that contains these compounds, supports bone differentiation of stem cells, and prevents bacterial adhesion. To evaluate our composite materials morphology, chemical structure, wettability, and thermal analysis were studied with different techniques. Anti-adhesive properties of the Streptococcus mitis (S. mitis) on composite materials were tested with bacterial colonization, and osteogenic inductive properties by human bone marrow mesenchymal stem cells (hBMSCs) with and without osteogenic differentiation medium were performed with cell viability assay, Alizarin Red staining, Ca deposition and SEM analysis. According to FESEM-EDS and ATR_FTIR data, the additives were highly incorporated into the PU nanofibers. The produced materials had randomly oriented nanofiber with a porosity of similar to 72 %. The FHP loaded nanofiber had an average diameter of similar to 185 nm. The swelling behavior of FHP-loaded PU roughly decreased for 3, 5, and 7 days due to the hydrophobic character of the sample. An increasing percentage of FHP decreased the bacterial colonization of S. mitis. PUn-CuCl2-FHP nanofibers did not exhibit any cytotoxic effect on the hBMSCs. According to Alizarin Red Staining and Ca deposition analysis, stimulation with both the material and the osteogenic medium triggered the bone differentiation of the hBMSCs in a combined manner at days-14. Based on the results, it can be deduced that natural fish head is a promising additive material for fabricating bone-like biomaterials.