Objectives: To evaluate the effect of renal denervation and serum nitric oxide level with a different time course of renal ischemia-reperfusion injury. Methods: Thirty-six male. Wistar rats were randomized into 6 groups. All rats underwent right nephrectomy to create a single kidney model. Renal denervated and innerved rats were subjected to renal clamping for 30-60 minutes. The study was performed in the Department of Anatomy, Akdeniz University, Antalya, Turkey, between June and November 2005. Results: Combined effect of denervation and ischemia may caused significant increase in serum nitric oxide levels and decrease in glomerular filtration rates. Conclusion: Our results indicate that kidney denervation did not cause any changes in renal functions, but with ischemia it worsens the deleterious effect of ischemia-reperfusion injury, and causes a significant increase in serum nitric oxide levels.