© 2019, Pakistan Botanical Society. All rights reserved.Turkey is one of the most outstanding places with the highest potential to grow barberry crop. The barberry possesses nutritional and health supplements and that can be grown naturally in almost all regions of Turkey. The goal of this paper was to determine chemical and phytochemical contents of barberry genotypes collected fromSivaslı district of Uşak province of Western Turkey. We identified 16 different barberry genotypes from the studied sites which were subjected to phytochemical analysis. We analyzed barberry juices in terms of chemical (fruit skin color, soluble solid content, acidity and pH) and phytochemical (antioxidant activity, phenol, flavonoids and ascorbic acids) values. The results indicated that total flavonoids contents were ranged from 261.66 to 965.97 mg CAT/100 g FW and the highest ascorbic acid values were found as 444.35 with the lowest ones as 120.36 mg/100 g. The antioxidant activity between genotypes was varied from 126.27 to 34.20% and total phenol contents were from 1198.53 to 2616.78 mg GAE/100 g FW. Statistical analysis showed that there was a significant difference (p<0.01) for all measurements between genotypes handled in this study and that a major correlation occurs between total antioxidant activity and total flavonoids, SSC, pH, L (brightness).