The effect of pretreatment or combined treatment of quercetin on menadione toxicity in rat primary mixed glial cells in vitro


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Oztopcu-Vatan P., KABADERE S., Uyar R.

CYTOTECHNOLOGY, vol.61, pp.11-16, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 61
  • Publication Date: 2009
  • Doi Number: 10.1007/s10616-009-9235-7
  • Journal Name: CYTOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.11-16
  • Keywords: Glia, Quercetin, Menadione, MTT, In vitro, INDUCED DNA-DAMAGE, OXIDATIVE STRESS, FLAVONOIDS, PROTECTION, BUTYLHYDROPEROXIDE, ANTIOXIDANT, METABOLITES, INHIBITION, ASTROCYTES, PROOXIDANT
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

Neurons and glia are highly susceptible to reactive oxygen species that play a key role in various neurodegenerative diseases. Menadione, a synthetic derivative of vitamin K, induces reactive oxygen generation. Quercetin one of the most ubiquitous bioflavonoids in food of plant origin, has strong antioxidant activities on different cell types, however recent studies demonstrated that it has also prooxidant and cytotoxic potentials. We examined the action of pre- and co-treatment of quercetin on menadione induced glial toxicity. The primary mixed glial cells obtained from 1 to 3 day old rat brain were pretreated with 10, 25, 100 or 250 mu M quercetin for 1 h, washed out and 10, 25, 50, 75 or 100 mu M menadione was added for 6 h. The other group of cells was treated with respective doses of quercetin combined simultaneously with the same doses of menadione for 6 h. The cells were washed and incubated for additional 24 h for recovery period and the viability was measured by using MTT assay. Menadione was dose-dependently toxic to glia cells and pretreatment with respective quercetin doses for 1 h could not eliminate this toxicity. Although 10 and 25 mu M quercetin combined with 10 and 25 mu M menadione could not change, 100 and 250 mu M quercetin together with 10 or 25 mu M menadione for 6 h increased further the menadione induced toxicity. We conclude that when combined with menadione, quercetin at high doses could be toxic to primary rat glia cells in culture.