Research Information System
30th Int Hort Congress (IHC) - Bridging the World through Hort / 5th Int Symposium on Plant Genet Resources - Sustainable Management and Utilizat for Food, Nutr and Environm Secur / Int Symposium on Appl Funct Mol Biol, İstanbul, Turkey, 12 - 16 August 2018, pp.367-375, (Full Text)
In this research it was aimed to characterize genetic relationship between 65 Coruh Valley pear genotypes and standard cultivars at molecular level. Pear genotypes were selected from Coruh Valley biodiversity between 2011 and 2012 within the scope of the project on Investigation of Fruit Genetic Resources in Eastern Anatolian Region, which started in 1994. These genotypes preserved in the Ex situ parcel in Erzincan Horticultural Research Station. Some international pear cultivars ('Santa Maria', 'Coscia', 'B PLMO', 'Williams' and 'Kieffer') and Turkish local pear cultivars ('Mustafabey', 'Limon', 'Ankara' and 'Deveci') were used as reference cultivars. SSR method was used for molecular characterization. SSR markers NH0021a, NH0027a, BGT23b, NH004A, KA4B and KA14 which were derived from pear genome and CH02d11, CH02b10, CH02f06 which were derived from apple genome were used because of their high polymorphism and descriptive properties. For 74 pear genotypes (including standard cultivars) used in the study, the average expected heterozygosity value was found 0.552, the average observed heterozygosity value was found 0.425 and the mean Shannon information index value was calculated as 0.892. CH02f06 locus was the most identifying locus (PIC: 0.557, Shannon information index: 1.073). Triple alleles were observed in 2 genotypes ('Arpa Pear', 'Boskova Pear'). The dendogram between genotypes was constructed according to UPGMA method. In the dendogram, 6 subgroups were formed. Two pairs of cultivars were identified to be synonyms and 4 pairs of cultivars to be homonymous. In this research genetic relationships between Coruh Valley pear germplasm and some important standard cultivars were determined. It is considered that the obtained data will contribute to the origin of pear cultivars from Coruh Valley, pear breeding programs and protection of genetic diversity.