© 2020 Bulgarian Academy of Sciences. All rights reserved.Up to now, thirteen Ponto-Caspian amphipod species belonging to seven genera have been recorded in Turkey. One of them is Pontogammarus robustoides which is known as an invasive Ponto-Caspian species in several European countries. The species was reported for the first time in Turkey in 1964, and since then has been found in 12 lakes and dam lakes located in the Marmara, Aegean and Thrace regions. In this study, materials collected during different study periods were examined and P. robustoides was detected at eight new localities: the lakes Akgöl and Poyrazlar, the reservoirs Demirköprü, Çayboğazı and Gördes, and the rivers Çekerek, Kocaçay and Bakırçay. In addition, P. robustoides is reported for the first time from the Central Anatolia and Mediterranean regions of Turkey. Our results indicate that this species has expanded its distribution area in many freshwater systems in Turkey. However, it has not been reported yet from eastern and southeastern regions of Turkey. Therefore, detailed studies dealing with distribution pattern and population status of this Ponto-Caspian amphipod are needed.