Natural environment and human relationships are very important in creating a healthy society. In particular, recent research focuses on the relationship between child and natural environments. Children's relationship with the natural environment could vary according to different variables. The gender factor is one of them. The aim of this present study was to determine the concern with regards to the natural environment of pre-adolescent living in Malatya city depending on gender. The study carried out a questionnaire survey that was given to the 6th, 7th and 8th form pupils of different primary schools based on children 11-14 years of age. A simple randomized sampling method was used to choose pre-adolescent who would complete the questionnaire form. The questionnaire that consisted of three sections - personal information', opinion about nature' and relationship with nature' - was given to 206 females, 170 males for a total 376 pre-adolescents. The results showed that the difference about opinion towards nature according to gender is not statistically significant. But the difference in leisure activities and relationship with nature is statically significant.