Secondary metabolites from Verbascum bugulifolium Lam. and their bioactivities

Gokmen A., Kusz N., Karaca N., DEMİRCİ F., Hohmann J., KIRMIZIBEKMEZ H.

NATURAL PRODUCT RESEARCH, vol.35, no.23, pp.5294-5298, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 23
  • Publication Date: 2021
  • Doi Number: 10.1080/14786419.2020.1753052
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, CINAHL, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.5294-5298
  • Keywords: antimicrobial, antioxidant and anti-inflammatory activities, Scrophulariaceae, secondary metabolites, Verbascum bugulifolium Lam
  • Eskisehir Osmangazi University Affiliated: No


Five iridoid glycosides catalpol (1), specioside (2), ajugol (3), ajugoside (4), 8-O-acetylharpagide (5), two phenylethanoid glycosides, verbascoside (6) and glucopyranosyl-(1 -> G(i)-6)-martynoside (7), four flavonoids, luteolin (8), luteolin 7-O-beta-glucopyranoside (9), luteolin 7-O-rutinoside (10), apigenin 7-O-rutinoside (11), quercetin 3-O-rutinoside (12) and beta-sitosterol (13) were isolated from the aerial parts of Verbascum bugulifolium Lam. for the first time. Their structures were elucidated by NMR and MS experiments. The extracts, and the isolates were evaluated for their in vitro antioxidant (DPPH center dot, ABTS center dot and CUPRAC), anti-inflammatory (LOX inhibition) and antimicrobial activities. Compounds 6 and 8 showed the highest antioxidant activity in all tests, where luteolin (8) showed the relatively best anti-inflammatory activity compared to other samples with 54.1 +/- 5.0% inhibition at 1 mu g/mL. All the tested compounds showed weak antimicrobial activity against tested microorganisms. This is the first phytochemical and bioactivity study on V. bugulifolium.