Genome-Wide Characterization of Expansin Gene Family in Cannabis sativa

Sipahi H.

ISPEC journal of agricultural sciences (Online), cilt.8, sa.1, ss.255-265, 2024 (TRDizin)

Özet

Expansins loose plant cell walls and play diverse roles in plant growth and development, germination, fruit ripening and softening, fiber development, and biotic/abiotic stress response, especially adaptation to the osmotic and oxidative stresses caused by drought stress. In this study, genome-wide analysis of the expansin gene family in Cannabis sativa was performed for the first time. A total of 29 expansin genes were found in the C. sativa genome. These genes were classified into four subfamilies, including 18 CsEXPAs, 5 CsEXPBs, 1 CsEXLAs, and 5 CsEXLBs family members. Phylogenetic analysis showed that the cannabis, Arabidopsis and rice expansins were divided into ten subgroups. 29 cannabis expansin genes were unevenly distributed among nine cannabis chromosomes. Most expansin genes have 3 exons while the number of introns and exons among expansin genes ranged from 1 to 4 and from 2 to 5, respectively. The promoter regions of 29 cannabis expansin genes contained diverse cis-elements that are involved in the development, environmental stress, hormones, and light responsiveness. This initial study is a useful resource for further research on the potential roles of expansin in fiber development, plant growth and development, and environmental stress response.