Influence of different co-cultivation temperatures, periods and media on Agrobacterium tumefaciens-mediated gene transfer

Uranbey S., Sevimay C., Kaya M. D., Ipek A., Sancak C., Basalma D., ...More

BIOLOGIA PLANTARUM, vol.49, no.1, pp.53-57, 2005 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 1
  • Publication Date: 2005
  • Doi Number: 10.1007/s10535-005-3057-z
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.53-57
  • Eskisehir Osmangazi University Affiliated: No


Tobacco leaf disc explants were inoculated with Agrobacterum tumefaciens strain GV2260 carrying p35S GUS-INT to determine the influence of different co-cultivation temperatures (18-26 degrees C), periods (24-96 h) and media (solid and liquid) on transformation efficiency. Kanamycin-resistant shoots developed on leaf discs inoculated with Agrobacterium after 4 weeks of culture initiation. Regenerated shoots were excised and rooted in the basal medium supplemented with 100 mg dm(-3) kanamycin and 250 mg dm(-3) augmentin. The rooted plantlets were finally transferred to compost and confirmed by GUS assay and PCR analysis. The highest transformation frequency was achieved from the explants co-cultivated with A. tumefaciens in liquid medium for 48 h at 22 or 24 degrees C.