Apoptosis induced by boric anhydrite (B2O3) after partial hepatectomy in rat liver

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Ozen A., Canbek M.

BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, vol.117, no.4, pp.231-234, 2016 (SCI-Expanded) identifier identifier identifier


Boron is one of the important elements that have a cell-growth suppressing effect. The apoptotic effects of B2O3 that were investigated in rats on liver regeneration following 70 % partial hepatectomy (PH). Wistar albino male rats were used and divided into 4 groups (n = 7). The Saline control groups were given only a single dose of saline; the B2O3-treated groups that were only given a single dose of 1800 nng.kg(-1) B2O3 by means of intraperitoneal injections following hepatectomy. Three and 6 hours after surgical procedures, all the groups were dissected and liver tissue samples were taken from the groups for NF-kappa B for caspase-3 gene and protein levels investigation by RT-PCR and TaqMan Protein Assay and histological analyses by TUNEL assay. B2O3-treated animals were examined and it was observed that NF-kappa B levels were decreased; however, caspase-3 gene expression and protein levels were increased significantly. This study demonstrated that B2O3 induces caspase-3 and inhibits NF-kappa B at the early stage of liver regeneration (Fig. 4, Ref. 26). Text in PDF www.elis.sk.