Acidolysis Reaction of Terebinth Fruit Oil with Palmitic and Caprylic Acids to Produce Low Caloric Spreadable Structured Lipid

Kocak D., Keskin H., Fadiloglu S., Gogus F.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, cilt.90, sa.7, ss.999-1009, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 90 Sayı: 7
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1007/s11746-013-2250-z
  • Dergi Adı: JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.999-1009
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

The lipase (Lipozyme IM from Rhizomucor miehei) catalyzed acidolysis reaction of terebinth (Pistacia terebinthus L.) fruit oil with caprylic and palmitic acid in hexane was investigated in a batch system. The effect of reaction conditions and relationship among them were analyzed and optimized by response surface methodology with a four-factor five-level central composite rotatable experimental design. The four major factors chosen were enzyme load (10-20 wt%), reaction time (12-20 h), reaction temperature (45-60 A degrees C) and substrate mol ratio (TO:PA:CA, 1:2.3-4.1:1.15-2.05). Optimum reaction conditions for reaction time, temperature, enzyme load and substrate mole ratio were 12 h, 45 A degrees C, 10 wt% and 1:4.1:2.05, respectively. The maximum yield of desired triacylglycerols (TAG) obtained at these optimum conditions was 50.87 %. Produced structured lipid had a caloric value which was 1.5 % lower than that of terebinth fruit oil. Its solid fat content was found comparable with commercially available margarines. The relative activity of lipase was well maintained in up to 10 repeated cycles.