This study aimed to analyze deaths related to tractor accidents in Eskisehir Province and characteristics of these cases to provide data and suggestions along with the literature. The cases involved individuals died due to tractor-related accidents were retrospectively examined between 1992 and 2016 (25-year period). Demographic data related to the cases and crime scenes, characteristics of events, and autopsy findings were evaluated. In the 25-year period, 61 individuals (88.5% males, average age: 48.7 years) died due to tractor accidents in Eskisehir Province, and approximately 45.9% of these accidents most commonly involved tractor rollover. Deaths due to tractor accidents will be decreased by raising awareness among tractor drivers, preventing young individuals from driving tractors, not allowing individuals to drive without license, making mechanical and physical features of tractors suitable for agricultural activity to be performed, ensuring their safety for travel and use, and avoiding carrying passengers on tractors.