Determination of rutin in Hypericum perforatum extract bu capillary electrophoresis

Dogrukol-Ak D., Kirimer N., Tuncel M., Aboul-Enein H.

ANALYTICAL LETTERS, cilt.34, sa.2, ss.185-191, 2001 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 2
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1081/al-100001569
  • Dergi Adı: ANALYTICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.185-191
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

A capillary zone electrophoretic (CZE) method for the determination of rutin in an ethanolic extract of the aerial parts of Hypericrum perforatum (Hypericaceae) is described. The analysis was performed using a fused-silica capillary and a background electrolyte consisted of 10% ethanol and 20 mM berate buffer at pH 8.0 applied at a potential of 28 kV, vacuum injection of 1 s and detecting at 200 nm. Rutin had a migration time of 10.1 +/- 0.2 min. Reproducibility was 1.26% for 9.37x10(-5) iM standard rutin. Detection limit was 2.7x 10(-6) M (S/N = 3). A well-correlated calibration equation was obtained for the mentioned conditions. The amount of rutin in the total plant material was found to be 0.21% +/- 0.02.