Cryogenic treatment is a supplementary heat treatment which can be applied to many different alloys. The effects of treatment are permanent and affect the entire material section. There have been many studies focused on the cryogenic treatment of tool steels. In the current study, an annealed Ti6Al4V material was used, and different cryogenic treatment processes and aging were applied. The effects of different heat treatment procedures on the microstructural and tribological properties were investigated. Eight different structures were obtained with different heat treatment cycles. As a result of wear tests and hardness tests, both cryogenic treatment and the aging treatment improved the wear resistance. The results of the ball-on-disk wear tests showed that the cryo-treated and aged samples have better wear resistance than annealed and shallow cryo-treated samples. 24 h deep cryogenic treatment improved wear resistance of the Ti6Al4V sample without significantly affecting hardness. It was also found that the aging process was effective due to both increasing hardness and wear resistance. Applying additional cryogenic treatment between solutionizing and aging improved this effect.