Scanning electron microscopic evaluation of the efficacy of 5% ethylenediaminetetraacetic acid for smear layer removal

ORHAN E. O. , Bahadir D., Akgun C., Aktas S.

MICROSCOPY RESEARCH AND TECHNIQUE, vol.84, no.2, pp.253-260, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 84 Issue: 2
  • Publication Date: 2021
  • Doi Number: 10.1002/jemt.23582
  • Page Numbers: pp.253-260


The purpose of the study was to evaluate the smear layer removal efficacy of novel Saver (R) irrigating solution including 5% EDTA and to compare the 17% EDTA as the most used counterpart. Forty-four single-rooted teeth were divided into four groups, were instrumented, and irrigated. The smear treatment groups were as follows: sterile distilled water; 5.25% sodium hypochlorite; Saver (R); 17% EDTA. The root samples were blinded. The micrographic images of root samples were collected by scanning electron microscope. The percentages of open dentin tubules were quantified using an image analysis software on micrographs. Two-way ANOVA followed by Tukey's multiple comparisons was used (p < .05). The 17% EDTA showed the highest percentages of open dentin tubules than the other treatments (p < .01). The mean percentages of open dentin tubules of 17% EDTA revealed statistically highly significant differences than Saver (R) treatment in all root regions (p < .01). Dentin tubules were covered by dense smear layer in distilled water irrigation whereas, the tubular pattern of dentin structure was not observed in hypochlorite irrigation. The commercial Saver (R) product presented to be less cleaning property on the smear layer than 17% EDTA and failed to show any improvement in the removal of smear layer compared with its tested counterpart.