Despite the rich biodiversity potential of Anatolia, the blackfly fauna of Turkiye has not been fully explored. In the present study, a sampling study for blackflies were performed in Western Black Sea Region in spring seasons from 2016 to 2018 and 15 blackfly species from 73 different lotic habitats have been reported. Simulium (Nevermannia) carthusiense Grenier & Dorier, 1959 is new for Turkish fauna. Ten species have been recorded from the region for the first time. Distributional remarks in Turkiye and the world are given along with brief taxonomic evaluations for each species.