Nasopharyngeal microbiota analysis of children with meningococcemia in pediatric intensive care unit: INMACS-PICU study

Creative Commons License

Bozan G., Aslan K., Kıral E., Perez Bocal V., Şevketoğlu E., Uysal Yazıcı M., ...Daha Fazla

11th CONGRESS OF THE WORLD FEDERATION OF PEDIATRIC INTENSIVE&CRITICAL CARE SOCİETES WFPIICS 2022, Cape-Town, Güney Afrika, 12 - 16 Temmuz 2022

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Cape-Town
  • Basıldığı Ülke: Güney Afrika
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Introduction: Microbiota composition might play a role on pathophysiology and course of sepsis and understanding the dynamics is of clinical interest. There is no information about the microbiota composition in children with meningococcemia. In this study, we plan to evaluate intestinal and nasopharyngeal microbiota composition of children with meningococcemia.

 

Material and Method: In this prospective, multi-center study, 10 children with meningococcemia (eight Men B, one Men W, and one non-groupable), and 10 age-matched healthy controls were included. Nasopharyngeal and fecal samples have been obtained at the admission to intensive care unit and Day 10. Detailed metagenomic analysis have been performed.

 

Results: Chao-1 and Shannon index in significantly lower in meningococcemia group, comparing the healthy controls (p<0.05). For beta diversity, while there is no difference between meningococcemia group and healthy controls at admission, there are difference between meningococcemia group at admission and Day 10. In meningococcemia group, Neisseria is the most abundant genus in the nasopharynx and we observed decreased abundance for Firmicutes and Cyanobacteria at phylum level, and Staphylococcus, Ruminoclostridium_5, Leptotrichia, Corynebacterium, Parabacteroides, Lactococcus, Bergeyella, Granulicatella, Intestimonas, Raoultibacter genera comparing the healthy controls. When we compared baseline and 10th day of PICU stay (after intensive antibiotic and supportive treatment), while Neisseria and Moraxella is the most abundant genera at admission, three days after abundance of 30 genera (Staphylococcus, Bacteroides, Desulfovibrio, Ruminoclostridium_5, Pseudomonas, Cutibacterium, Alistipes, Fusobacterium, Parabacteroides, Corynebacterium, Elusimicrobium, Oxalabacter, Psycrobacillus, Novosphingobium, Desulfatifurela, Tannerella, Anaerosporobacter, Enterobacter, Odoribacter, Enterococcus, Akkermansia, Paludibacter, Azorhizophilus, Lachnoclostridium, Bifidobacterium, Serratia, Tepidimonas, Geobacillus, Raoultibacter).

 

Conclusion: At the time of admission, nasopharyngeal microbiota composition in patients with meningococcemia was found to be significantly different from healthy children. At the 10th days of admission, majority of the normal nasopharyngeal microbiota members returned to be normal with an antibiotic and other supportive treatment. This is the first study showed the alterations of microbiota composition in children with meningococcemia.