Objective: We aim to investigate the benefit of some important markers in predicting pregnancy of unexplained infertility cases. These markers are important in implantation, oocyte quality, embriyonic development of in vitro fertilization-embriyo transfer (TVF-ET) cycles. Material and Methods: This prospective cohort study was conducted on 40 infertile couples with unexplained infertility. All couples had at least two unsuccesfull superovulation+intrauterine insemination treatment cycle history. Assisted reproductive technology (ART) cycle start (days 2-3) and oocyte pick-up (OPU) day serum samples and received follicle fluids taken from all women, glikodelin, vascular endothelial growth factor (VEGF) and interleukin-1β (IL-1β) levels examined by Enzyme-Linked Immunosorbent Assay (ELISA) method. We studied to determine the relationship between these markers and clinical pregnancy rate. Results: According to the results there is no significant association in group according to pregnancy results between days 2-3, OPU day serum samples and follicular fluid VEGF, IL-1β and glycodelin levels (p>0.05). Conclusion: We concluded that further studies with large series are needed to reveal the parameters that we studied could be used in evaluation of patients with unexplained infertility. Copyright © 2014 by Türkiye Klinikleri.