Akademik Veri Yönetim Sistemi
MEDITERRANEAN AGRICULTURAL SCIENCES , cilt.37, sa.1, ss.51-55, 2024 (Hakemli Dergi)
Global warming is becoming a greater threat for the agricultural sector, while molecular genetics
studies still hold new opportunities, to not only detect heat-tolerant animals, but also to allow
for increasing the frequencies of desired genotypes in a certain population. In this study,
HSP90AB1 gene associated with heat tolerance was investigated in four cattle breeds known as
Zavot (ZAV), Sout Anatolian Yellow (SAY), South Anatolian Red (SAR), and Brown Swiss
(BS) reared in Türkiye via Allele-Specific Polymerase Chain Reaction (AS-PCR). 4338T>C
mutation of the HSP90AB1 gene yielded a total of three genotypes (CC, CT, and TT) across all
cattle breeds in which C allele frequency ranged from 0.34 (SAY) to 0.73 (BS), while T allele
frequency varied between 0.27 (BS) and 0.66 (SAY). In BS, CC was the genotype with the
highest frequency (0.50), whereas the frequency of CC was lower than CT and TT in the
Anatolian breed (ZAV, SAR, and SAY). Similarly, the frequency of TT was higher in native
Anatolian breeds than BS (0.05). All the populations studied were in Hardy-Weinberg
Equilibrium (HWE) in terms of the HSP90AB1 gene. This study confirmed that the HSP90AB1
gene was polymorphic in four cattle breeds reared in Türkiye. This polymorphism has the
potential to allow for improving heat tolerance to maintain animal production in the future via
suitable selection studies. Therefore, this polymorphism should be conserved in Anatolian cattle
breeds, while other genes related to different environmental stressors may be monitored by
further studies