Prognostic value of midkine, syndecan-1, hyaluronan synthase-2, sestrin-1, laminin subunit alpha-4 and fibulin-3 for malignant pleural mesothelioma.

Akgun H., Metintas S., Ak G., Demirkol Canlı S., Isbilen M., Gure A. O., ...Daha Fazla

Archives of medical science : AMS, cilt.19, sa.2, ss.355-364, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.5114/aoms/112525
  • Dergi Adı: Archives of medical science : AMS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.355-364
  • Anahtar Kelimeler: prognosis, mesothelioma, midkine, sestrin-1, hyaluronan synthase-2, syndecan-1, fibulin-3, laminin subunit alpha-4, STAGING PROJECT PROPOSALS, FORTHCOMING 8TH EDITION, TNM CLASSIFICATION, IASLC MESOTHELIOMA, CANCER, DESCRIPTORS, EXPRESSION, BIOMARKER, SURVIVAL, DIFFERENTIATION
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

Introduction: The prognosis of malignant pleural mesothelioma (MPM) is poor, with a limited survival time. In this study, we aimed to examine expres-sion levels of genes selected from relevant literature and to utilize in silico methods to determine genes whose expression could reflect the prognosis of patients with MPM by ex-vivo validation experiments. Material and methods: The study group consisted of 54 MPM patients treated with chemotherapy. Expression of 6 genes - midkine (MDK), syndecan-1 (SDC1), hyaluronan synthase-2 (HAS2), sestrin-1 (SESN1), laminin subunit alpha-4 (LAMA4), and fibulin-3 (FBLN3) - was examined by qPCR in tumor tissues. Sestrin-1 and LAMA4 were identified using an in house R-based script: Unsupervised Sur-vival Analysis Tool. Midkine, SDC1, HAS2, and FBLN3 were selected from cur-rent literature. We used two housekeeping genes, i.e. glucose-6-phosphate dehydrogenase and TATA-box binding protein, as controls. Results: Of the patients, 43 (79.6%) had epithelioid mesothelioma. The me-dian survival for all patients was 10 (+/- 1.2 SE) months (95% CI: 7.7-12.3). In multivariate analyses, MDK (p = 0.007), HAS2 (p = 0.008) and SESN1 (p = 0.014) expression levels were related to survival time in the whole group. In epithelioid type MPM patients, MDK (p = 0.014), FBLN3 (p = 0.029), HAS2 (p = 0.014) and SESN1 (p = 0.045) expression was related to survival time in multivariate analyses. Conclusions: High HAS2 and SESN1 expressions and low MDK are potential biomarkers of good prognosis in MPM. High HAS2 and SESN1 expression and low MDK and FBLN3 can also be utilized as biomarkers of good progno-sis for epithelioid MPM. Those results should be further investigated in sera, plasma, and pleural effusions.