Akademik Veri Yönetim Sistemi
2nd International Silkroad Congress, Iğdır, Türkiye, 26 - 27 Eylül 2023, ss.110-115
Seventy-one diseased
plant samples were taken from turfgrass areas during the surveys in Antalya
province, Türkiye in 2015. Dead spot symtoms were observed in five golf courses
during the surveys conducted in Belek region, in Antalya. On golf courses where
the agent is isolated, collapsed small patches in the form of whitish yellow
circles 10-12 cm in size were seen. When the roots, stolons and rhizomes of the
diseased plants were examined, it was observed that they were browned and
rotten. As a result of isolations from infected roots on potato dextrose agar
(PDA) supplemented with 100 mg/liter streptomycin sulfate, a fungus with aerial
mycelial developed which was light gray at first and turned dark gray over
time. As a result of isolations from turfgrass diseases plants, obtained
isolates were identified with the rDNA-ITS sequences analysis using universal
primer pair ITS1/ITS4. The ITS1-5.8S-ITS2 region of isolates were sequenced and
performed BLAST analyzes. Five isolates were identified as Ophiosphaerella
korrae. Pathogenicity tests were performed on 8 week old C. dactylon
variety grown in plastic pots (12 × 12 cm). The substrate (sandy soil and
perlite) was infested separately with five isolates of O. korrae grown
on autoclaved wheat kernels, using 10 infested kernels per pot. As a result of
the pathogenicity tests on Petri dishes and pots, diseases severity value of
the isolates in Petri dishes were range
from 92%- 98%, were range from in pots 82%- 95% . This is the first report of Ophiosphaerella
korrae causing root rot of turfgrass areas in Türkiye.