Aquatic pollution caused by synthetic dyes poses a significant threat to environment and public health. Efficient and convenient removal of dye contaminants from aquatic environment is a challenge for environmental protection. Biosorption process is a promising way to remove such chemicals from contaminated media. Herein, a new biomagsorbent based on Lactarius salmonicolor cells (MagLS) was simply designed by combining magnetic separation and biosorption techniques. Technical feasibility of the prepared biomagsorbent for Reactive Yellow 2 retention was examined. The interactive effects of batch process variables were evaluated by 4 level Box-Behnken design. Kinetic analysis indicated applicability of the pseudo second order model. q(max) value was noted as 115.23 mg/g and retention of RY2 was endothermic and spontaneous in nature. IR, SEM/EDX analysis and zeta potential measurements were employed for the characterization. Flowthrough experiments indicated that MagLS has a high decolorization potential. Regeneration experiments carried out in 25 consecutive cycles revealed that MagLS can be easily regenerated and reused for at least 15 cycles with high sorption (similar to 70%) and desorption (similar to 80%) yield. Furthermore, after 25 cycling process, a recovery yield of RY2 dye was maintained at around 80%. (C) 2018 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.