© 2022 Elsevier B.V.Solid lipid nanoparticles (SLN) have many various properties such as controlled drug release, elimination of side effects of drugs, etc. In this study, drug release study of SLN formulations was performed with Nuclear Magnetic Resonance (NMR) spectroscopy. Results obtained from NMR experiments were compared with other studies in the literature in which drug release of doxorubicin encapsulated in SLN formulations was carried out using High Pressure Liquid Chromatography (HPLC) and UV-VIS spectrophotometer. In NMR experiments, d6-DMSO and tetra methyl silane (TMS) were used as solvent and internal standard materials, respectively. Moreover, ferrocene was used as internal standard material in qNMR experiments. Doxorubicin was encapsulated in SLNs and after investigating the physicochemical properties of SLN formulations, specificity and selectivity of the qNMR method were evaluated by ratio of S/N ratio (signal/noise ratio) of singlet signal of doxorubicin to ratio of S/N ratio signal of ferrocene (internal standard material). Moreover, accuracy and precision of the qNMR method were evaluated. Results of qNMR method were validated. The results obtained from the qNMR method have been shown to be compatible with the results obtained from other traditional methods such as HPLC, UV-VIS, etc. It was also shown that the qNMR method is a simple and easy method for release of drugs encapsulated in SLNs. As a result, qNMR method is a suitable characterization method for determination of drug release of SLN formulations.