Akademik Veri Yönetim Sistemi
3rd International Eurasian Mycology Congress, Van, Türkiye, 7 - 09 Eylül 2022, ss.1-2
Many
fungal species are part of the natural microbiota, which has an important role
in human health. However, when the immune system is compromised, commensal
fungal species can transform into opportunistic pathogens that systematically
migrate to develop invasive fungal infections that affect multiple systems. Natural
bioactive molecules that can be used against these pathogens are often
synthesized by filamentous microfungi. Silver nanoparticles (AgNP) with unique
properties such as shape, size and increased surface area are known to indicate
antimicrobial properties. Combining biogenic AgNP with other bioactive
metabolites is a promising new strategy to control resistant infections.
In this study, ethyl acetate extract was
obtained from P. griseofulvum E7P, which was isolated and identified
from İzmir Çamaltı Saltern, and the fractions of this extract purified by
column chromatography were used. The antifungal activities of the purified
fractions (A-F) and AgNP were determined by microdilution method. The
checkerboard method was used to determine the synergistic activities of fungal
extracts and AgNP on yeast and dermatophytes. The Fractional Inhibition
Concentration value was calculated for each well that did not grow at the end
of the incubation period. This value was used to determine the effect type.
The MIC value of Fraction D against C.
albicans and C. krusei was found to be 200 µg/ml, and Fraction F
against dermatophytes as 15.6 µg/ml. AgNP MIC values were in the range of
25-1.56µg/ml against both test microorganism groups. The synergy and partial
synergy effects obtained from the combinations examined in synergistic activity
studies give hope in the fight against resistant pathogens.
Keywords: Fungal
metabolite, Biogenic AgNP, Dermatophyte, Synergetic activity