The purpose of this research was to determine the biochemical contents of black (Morus nigra L.) mulberry genotypes grown in the Kutahya region. Total soluble solids content, pH, titratable acidity, total phenolics, vitamin C, DPPH radical scavenging activity, and soluble sugars (fructose, glucose, and sucrose) of black mulberry genotypies were determined at the end of the study. The highest total phenolics value was detected in the SIM02 (2995.16 mg GAE g-1). The SIM03 genotype had the highest vitamin C content of 31.34 mg 100 g-1. The study indicated that radical scavenging activity (DPPH) 19.05 (SIM03) as the highest. In terms of the most valuable chemical composition, the genotypes SIM01, SIM02, and SIM03 can be suggested and used for future breeding reasons. It is desirable to take actions in Turkey to conduct an extensive conservation program for Morus nigra L. biodiversity.