Purification of the phytase enzyme fromLactobacillus plantarum: The effect on pansy growth and macro-micro element content


PARLAKOVA KARAGÖZ F., Demir Y., Kotan M. S., DURSUN A., BEYDEMİR Ş., DİKBAŞ N.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.68, sa.5, ss.1067-1075, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/bab.2026
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1067-1075
  • Anahtar Kelimeler: enzyme purification, phytase, plant growth, Viola × wittrockiana
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

In the present study, the phytase enzyme was purified fromLactobacillus plantarumwith a 3.08% recovery, 9.57-purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro-micro nutrient content of pansy (Violaxwittrockiana), which is of great importance in ornamental plants industry. The research was established under greenhouse conditions with natural light in 2017. The pansy seeds were coated with phytase enzyme solution, sown in a peat environment, and transferred to pots at the seedling period. In general, the 5 EU and 10 EU applications increase plant height, the number of leaves per plant, the number of side branches per plant, and flower height parameters compared to control. Also, micro- and macronutrient values in soil and plant samples were examined. According to the results, the phytase application on pansy cultivation positively affected the properties and yielded high quality of plants.