Effect of agitation of EDTA with 808-nm diode laser on dentin microhardness

Arslan H., Yeter K., KARATAŞ E., Yilmaz C. B., Ayranci L. B., Ozsu D.

LASERS IN MEDICAL SCIENCE, vol.30, no.2, pp.599-604, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1007/s10103-013-1364-8
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.599-604
  • Eskisehir Osmangazi University Affiliated: No


The aim of this study is to analyze the effect of agitation of ethylenediaminetetraacetic acid (EDTA) with diode laser at different agitation times on root dentin microhardness. Eighty-four specimens were divided randomly into seven groups, as follows: (1) distilled water, (2) 17 % EDTA, (3) EDTA with 60 s ultrasonic agitation, (4) EDTA with 10 s laser agitation, (5) EDTA with 20 s laser agitation, (6) EDTA with 30 s laser agitation, and (7) EDTA with 40 s laser agitation. All of the specimens were irrigated with 5 % NaOCl and distilled water except the distilled water group. Microhardness values were calculated before and after the procedures. Statistical analyses were performed using one-way ANOVA and Tukey post hoc tests. Statistically significant differences were determined between the distilled water and other groups. Also, statistically significant differences were observed between EDTA with 40 s laser agitation and EDTA, and EDTA with 10 and 20 s laser agitations. Ultrasonic agitation of EDTA affected microhardness of root dentin similar to EDTA (p > .05). All applications decreased the microhardness of root dentin when compared with distilled water. Agitation of EDTA with diode laser for 40 s caused more reduction in microhardness of root dentin when compared with EDTA.