Cytotoxic Effects of Different ICG Concentrations and Laser Parameters on Neuroblastoma

AK CAN A., Kaya O., TURGUT COŞAN D., Gulsoy M.

ACTA PHYSICA POLONICA A, vol.128, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 128
  • Publication Date: 2015
  • Doi Number: 10.12693/aphyspola.128.b-381
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Eskisehir Osmangazi University Affiliated: Yes


Photodynamic therapy (PDT) is a minimally invasive treatment for cancer therapy. It can be administered in combination with other treatments such as chemotherapy, radiotherapy, and surgical excision. PDT involves a photosensitizing agent that is activated by exposure to a specific wavelength of light. PDT is a cold photochemical process, there is no tissue heating. In our study, we investigated whether different laser parameters with different concentrations of indocyanine green (ICG) have cytotoxic and anti-proliferative effects on neuroblastoma. Plates were divided groups as control, only ICG concentrations (25 and 5 0 mu g/ml), only laser treatment I (50 J/cm(2)), only laser treatment II (100 J/cm(2)), 25 mu g/ml ICG + laser treatment I and 2 5 mu g/ml ICG + laser treatment II, 5 0 mu g/ml ICG + laser treatment I and 5 0 mu g/ml ICG + laser treatment II. Neuroblastoma cell lines were irradiated with an in-house developed diode laser system (lambda = 8 0 9 nm, 70 mW/cm(2), 50 & 100 J/cm(2)) in continuous wave operation mode after ICG application. Cell proliferation was measured by XTT assay after light irradiation. Cell proliferation was decreased in a dose-dependent manner in 25 and 5 0 mu g/ml ICG concentrations when compared with control. The applied ICG concentrations (especially 5 0 mu g/ml) had cytotoxic effects for neuroblastoma cell lines, SH-SY5Y. There was no difference between laser treatment groups (L 50 & 100 J/cm(2)). However, PDT groups (laser exposure with ICG) showed significant inhibition of cell viability (p < 0 : 0 5). Additionally, laser exposure did not increase the well temperature above the incubation parameter. In conclusion, PDT has cytotoxic effects in neuroblastoma cell lines. Appropriate ICG dose - laser parameter combinations must be determined for each cell type. Different energy densities may cause different effects of PDT on inhibition of cell viability.