Immunological approaches to plant cell wall and biomass characterization: Glycome profiling

Pattathil S., Avci U., Miller J. S., Hahn M. G.

Methods in Molecular Biology, vol.908, pp.61-72, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 908
  • Publication Date: 2012
  • Doi Number: 10.1007/978-1-61779-956-3_6
  • Journal Name: Methods in Molecular Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.61-72
  • Keywords: Biomass, Cell wall glycans, ELISA, Glycome profiling, Heatmap, Monoclonal antibody
  • Eskisehir Osmangazi University Affiliated: No


The native complexity of plant cell walls makes research on them challenging. Hence, it is advantageous to have a diversity of tools that can be used to analyze and characterize plant cell walls. In this chapter, we describe one of two immunological approaches that can be employed for screening of plant cell wall/biomass materials from diverse plants and tissues. This approach, Glycome Profiling, lends itself well to moderate to high-throughput screening of plant cell wall/biomass samples. Glycome Profiling is being further optimized to reduce the amount of sample required for the analysis, and to improve the sensitivity and throughput of the assay. We are optimistic that Glycome Profiling will prove to be a broadly applicable experimental approach that will find increasing application to a wide variety of studies on plant cell wall/biomass samples. © 2012 Springer Science+Business Media, LLC.