Genetic relationships among some Turkish Petrorhagia (Ser.) Link (Caryophyllaceae) taxa using ISSR markers

Hilooglu M., Eröz Poyraz İ., Poyraz İ., Ataşlar E., Sözen E.

PHYTOTAXA, cilt.272, sa.2, ss.165-172, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 272 Sayı: 2
  • Basım Tarihi: 2016
  • Doi Numarası: 10.11646/phytotaxa.272.2.8
  • Dergi Adı: PHYTOTAXA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.165-172
  • Anahtar Kelimeler: Petrorhagia, Genetic relationship, ISSR, Turkey, PHYLOGENETIC-RELATIONSHIPS, IBERIAN PENINSULA, DIVERSITY, EVOLUTIONARY, DNA
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

A study of the genetic relationships among Petrorhagia taxa from Turkey was carried out using inter-simple sequence repeat (ISSR) markers. A total of 409 amplified bands were obtained by 10 ISSR primers. The polymorphism ratio was high (100%) across 45 individuals representing nine Petrorhagia taxa (P. dubia, P. prolifera, P. pamphylica, P. peroninii, P. saxifraga, P. cretica, P. alpina subsp. alpina, P. alpina subsp. olympica, P. lycica) and was sufficient to distinguish each species. Statistical analyses were performed by using POPGENE, GenAlEx6, and PAUP. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram was constructed based on Nei's genetic distance along with outgroup species (Velezia rigida) in MEGA4. The dendrogram shows two main clusters, the first one (Cluster-I) included only P. lycica, while the cluster-II contained all other taxa. Cluster-II can be grouped in two sub-clusters, with P. prolifera and P. saxifraga constituting a first sub-cluster, the other species (P. alpina subsp. alpina, P. alpina subsp. olympica, P. cretica, P. dubia, P. peroninii and P. pamphylica) being grouped in a second sub-cluster. Both PCoA and Neighbour-Net network analysis supported the dendrogram. The study showed that ISSR technique can be successfully used in species identification and determination of the genetic relationships between Petrorhagia species distributed in Turkey.