Akademik Veri Yönetim Sistemi
INFLAMMATION, cilt.35, sa.2, ss.420-428, 2012 (SCI-Expanded)
Bradykinin, a vasoactive peptide, increases during inflammation and induces the formation of prostaglandins through specific receptor activation. Two types of receptors mediate the biological effects of bradykinin, B-1 and B-2 receptors. Although B2 receptors are present in most tissues, B-1 receptors are expressed after inflammatory stimuli or tissue injury. Bradykinin has a high affinity for B-2 and a low affinity for B1 receptors, whereas the opposite occurs for des-Arg(9)-bradykinin. Recently, it has been reported that nonsteroidal anti-inflammatory drugs have different inhibitory activities on cyclooxygenase isozymes, COX-1, COX-2, and COX-3. In the present study, we have investigated the contributions of different COX isozyme inhibitions and inflammation on bradykinin-induced effects of isolated rat aorta and urinary bladder smooth muscle contractions. Male Sprague-Dawley rats weighing 200-250 g were used in the study. The vasodilatory responses to bradykinin (1 nM-1 mu M) were studied on isolated rat aorta rings contracted with norepinephrine (0.1 mu M) following incubation with dipyrone (100, 700, and 2,000 mu M). The relaxant responses of dipyrone (100, 700, and 2,000 mu M) were also compared on the isolated rat urinary bladder contracted with bradykinin (n=8). A bacterial lipopolysaccharide was used for the induction of inflammation (n=8). The levels of PGE(2), PGF(1 alpha), TXB2, nitric oxide synthase (NOS), IL-10, and TNF-alpha were all determined in both the plasma and the perfusate of the aorta preparations (n=5). The vasodilatory activities of bradykinin and des-Arg9-bradykinin were significantly increased upon the inhibition of COX-3 (dipyrone at 100 mu M). These effects disappeared in the inflamed group. PGE(2), PGF(1 alpha), and TXB2 were significantly high, but NOS activity was low in the aorta perfusate after the inhibition of COX-3. Dipyrone showed the relaxant activity of the urinary bladder contracted with bradykinin. The vasodilatory activity of des-Arg(9)-bradykinin was in the inflamed group but not in the non-inflamed group. Bradykinin did not contract urinary bladder in inflamed group. The results suggest that COX-induced products may play an important role in the bradykinin-induced rat aortic smooth muscle relaxations.