Quercetin decreases sterile inflammation proteins NLRP3 and caspase 1 in clone-9 cell line damaged by hydrogen peroxide

ŞAHİN E., Dabagoglu Psav S., ŞAHİNTÜRK V., Koparal A. T.

RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI, vol.32, no.4, pp.911-919, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.1007/s12210-021-01031-y
  • Journal Name: RENDICONTI LINCEI-SCIENZE FISICHE E NATURALI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.911-919
  • Keywords: Quercetin, Oxidative stress, Sterile inflammation, NLRP3 inflammasome, Clone 9 liver cell, INDUCED OXIDATIVE STRESS, FATTY LIVER-DISEASE, EPIGALLOCATECHIN GALLATE, DNA-DAMAGE, ANTIOXIDANT, METABOLISM, APOPTOSIS, RUTIN, CYTOTOXICITY, POLYPHENOLS
  • Eskisehir Osmangazi University Affiliated: Yes

Abstract

NLRP3 inflammasome is very important in liver diseases and is intimately linked to oxidative stress. Quercetin is a significant antioxidant and anti-inflammatory biological molecule. The aim of current study was to investigate the effects of quercetin on oxidative stress and NLRP3 and caspase-1 proteins involved in sterile inflammation induced by H2O2 in clone-9 cells. MTT, DCF-DA assays, immunocytochemical and fluorescence staining methods were used. After 24 h incubation with H2O2, the IC50 was found to be 278 mu M in clone-9 cells. The most effective dose of quercetin beside H2O2-associated damage was found to be 15 mu M. It was shown that 278 mu M of H2O2 caused pyknosis in cell nuclei, while 15 mu M of quercetin protected the cells from the pyknosis. Quercetin pretreatment was shown to reduce H2O2-induced ROS production significantly (p < 0.05). It was found that NLRP3 and caspase-1 immunoreactivity were increased in H2O2-treated groups and quercetin pretreatment reduced the expression of these proteins. According to the results, quercetin reduceses sterile inflammation proteins and H2O2-induced oxidative stress in clone-9 liver cells. Quercetin may be therapeutic in the treatment of liver and NLRP3 inflammasome based diseases. Quercetin can also be taken with food for prophylactic purposes.