Therapeutic Potential of Marrubium alysson L. Extract: Dose-Dependent Cytotoxicity in HNO210 Human Laryngeal Carcinoma Cells
Journal of Craniofacial Surgery, 2026 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Publication Date: 2026
- Doi Number: 10.1097/scs.0000000000012574
- Journal Name: Journal of Craniofacial Surgery
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, MEDLINE
- Keywords: Cell morphology, cell viability, cytotoxic effect, HNO210 laryngeal carcinoma cells, Marrubium alysson L. extract, MTT assay
- Eskisehir Osmangazi University Affiliated: Yes
Abstract
Objectives: – We investigated the concentration-dependent cytotoxic effects of Marrubium alysson L. extract on HNO210 human laryngeal carcinoma cells. Methods: – The HNO210 human laryngeal carcinoma cell line (BHC11100312, BioHippo) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) (ATCC, 30-2002). Seven working concentrations (10–500 μL/mL) of Marrubium alysson L. were prepared. The antiproliferative effect was assessed using the colorimetric MTT assay. Results: – Marrubium alysson L. inhibits the viability of HNO210 cells. The corresponding logIC₅₀ (2.681 μL/mL), along with a high coefficient of determination (R²=0.9960), confirms the reliability of the dose-response relationship. While lower concentrations largely preserve cell viability, higher doses significantly suppress metabolic activity. Marrubium alysson L. extract demonstrates a concentration-dependent cytotoxic effect on HNO210 cells. After 24 hours of exposure, treated cells showed notable morphologic changes, including cell shrinkage, rounding, and detachment from the culture surface, indicating loss of membrane integrity and decreased adherence. Conclusion: – Marrubium alysson L. inhibits the viability of HNO210 laryngeal carcinoma cells. It exerts a concentration-dependent cytotoxic effect and anticancer potential against these cells. It affects cell morphology and reduces adherence. Marrubium alysson L. has potential as a source of bioactive compounds with antiproliferative activity for designing highly effective anticancer drugs.