In removing of suspended particles from wastewater, settling methods becomes insufficient when the suspensions are diluted and contain fine/ultrafine and colloidal particles. In that case, floating of suspended particles by applying floc-flotation is faster than settling methods and the floated sludge contains higher solid content. In this study, the Jameson flotation cell which is an improved version of induced air flotation was used to remove suspended particles. Flocculation-flotation (Floc-flotation) methods were applied together to increase the efficiency of wastewater treatment. Some of the critical variables (Feed flow rate, flotation duration, and conditioning time) that directly impact flotation performance were examined. One of the most popular response surface methodologies (RSM), Central Composite Design (CCD), which has three parameters and five levels, was utilized to plan and conduct the experiments. By measuring the residual turbidity of clarified water and particle removal recovery, the efficacy of the floc-flotation process in the Jameson cell was assessed. The results showed that flotation time and feed flow rate have a significant impact on both residual turbidity and flotation recovery than conditioning time. Interaction effects between feed flowrate and flotation time and between flotation time and conditioning time were also found significant in both responses. The optimal condition that aiming the lowest turbidity while maximizing flotation recovery was identified. The results of this study indicate that floc-flotation applied with Jameson cell is an effective method for removing very fine particles from extremely diluted concentrations.