The gubernacular canal or gubernacular tract is filled by the gubernacular cord, which includes fibrous connective tissue containing peripheral nerves, blood and lymphatic ducts besides the epithelial cells from the fragmented dental laminae, including epithelial growth factor. The purpose of this study was to evaluate the gubernacular tract in unerupted supernumerary teeth by cone beam computed tomography. Sixty-four unerupted supernumerary teeth were selected from 44 patients (21 females, 23 males, 12-68 years). Gubernacular tract characteristics were evaluated in five different groups: No alteration, bending of gubernacular tract, contraction of gubernacular tract, obliterations of gubernacular tract, difference between erupted direction. Unerupted supernumerary teeth were classified according to their position. The presence and characteristics of the gubernacular tract in the supernumerary teeth were evaluated by cone beam computed tomography.