RAPID ANALYSIS OF VERAPAMIL IN PLASMA BY REVERSED-PHASE HPLG


YAZAN Y., BOZAN B.

PHARMAZIE, vol.50, no.2, pp.117-119, 1995 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 2
  • Publication Date: 1995
  • Journal Name: PHARMAZIE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.117-119
  • Eskisehir Osmangazi University Affiliated: No

Abstract

A modified and simple HPLC procedure has been developed for verapamil in plasma. Plasma samples have been vortex-mixed and centrifuged without any need of extraction. The analysis has been performed on a C-20 reversed-phase column with fluorometric detection using 5,6-benzoquinoline as an internal standard. Standard curve has been found to be Linear for concentrations from 30 to 1000 ng/ml (plasma) for verapamil and no potential source of interference was present. The method has the advantages of speed, small sample requirement and reproducibility. Applicability of the method has been demonstrated by a pharmacokinetic study in 7 rabbits which received a single dose of 0.25 mg verapamil hydrochloride by intravenous administration.