RAPID ANALYSIS OF VERAPAMIL IN PLASMA BY REVERSED-PHASE HPLG


YAZAN Y., BOZAN B.

PHARMAZIE, cilt.50, sa.2, ss.117-119, 1995 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 2
  • Basım Tarihi: 1995
  • Dergi Adı: PHARMAZIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.117-119
  • Eskişehir Osmangazi Üniversitesi Adresli: Hayır

Özet

A modified and simple HPLC procedure has been developed for verapamil in plasma. Plasma samples have been vortex-mixed and centrifuged without any need of extraction. The analysis has been performed on a C-20 reversed-phase column with fluorometric detection using 5,6-benzoquinoline as an internal standard. Standard curve has been found to be Linear for concentrations from 30 to 1000 ng/ml (plasma) for verapamil and no potential source of interference was present. The method has the advantages of speed, small sample requirement and reproducibility. Applicability of the method has been demonstrated by a pharmacokinetic study in 7 rabbits which received a single dose of 0.25 mg verapamil hydrochloride by intravenous administration.