Influences of uterine tubal factors on oocyte maturation and embryo segmentation

GÜRER F., ŞAHİNTÜRK V., BAYÇU C., AÇIKALIN A. E.

GORM:Gynecology Obstetrics & Reproductive Medicine, cilt.10, sa.1, ss.39-43, 2004 (Hakemli Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 1
  • Basım Tarihi: 2004
  • Dergi Adı: GORM:Gynecology Obstetrics & Reproductive Medicine
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.39-43
  • Eskişehir Osmangazi Üniversitesi Adresli: Evet

Özet

OBJECTIVE: The aim of this study was to investigate the effects of uterine tubal epithelium or its fluid on in vitro oocyte maturation and embryo development of rats. STUDY DESIGN: For this purpose, mature, immature cumulus oocyte complexes (COC) and embryos from wister albino rats were used. Mature and immature COC of control groups were incubated in 10% EBSS (Earle's Balanced Salt Solution) and control embryos were cultured in 15% EBSS. Test groups were cultured with same culture mediums in which uterine tubal fluid or uterine tubal epithelium was added. Survivals of oocytes and embryos were evaluated with a phase-contrast inverted microscope. In vitro maturation of oocyte and embryo segmentation were observed with light, fluorescence and transmission electron microscopes. RESULTS: When uterine tubal epithelium or its fluid were added to the conventional culture medium, the maturation of immature oocytes was increased and the segmentation ratios of embryos was accelerated. CONCLUSIONS: Addition of uterine tubal epithelium or its fluid to the conventional medium as a source of various endogenous mediators bringing out more alikeness in vivo conditions may play an important role in paracrine communications for better maturation of oocytes and viabilities of pre-implantation embryos.